PRIMER PICKING RESULTS FOR thermod_default

No mispriming library specified
No internal oligo mishyb library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%  any_th  3'_th hairpin seq
LEFT PRIMER         11   20   59.70   55.00    0.00   0.00    0.00 GACTTCACTTTCCGACCCTG
RIGHT PRIMER       113   20   59.96   50.00    9.59   0.00    0.00 TGACGGTCATCATGGTGAGT
INTERNAL OLIGO      44   20   60.71   60.00    0.00   0.00   40.53 CACCCACGAGTGAAGACTCC
SEQUENCE SIZE: 178
INCLUDED REGION SIZE: 178

PRODUCT SIZE: 103, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

    0 AAGCCGAGGATGACTTCACTTTCCGACCCTGCAGAGCCGAGTTTCACCCACGAGTGAAGA
                 >>>>>>>>>>>>>>>>>>>>             ^^^^^^^^^^^^^^^^

   60 CTCCGCCAGCCTTGCCCCTCGGGCCTCTCCCTCAACTCACCATGATGACCGTCAGGCTGA
      ^^^^                              <<<<<<<<<<<<<<<<<<<<      

  120 GGAGGCAGGCGGACTGAGGCGTGGTCCGCGGGGCTCAGGGAGGTGGCTCCTGCGCCGG
                                                                

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo

ADDITIONAL OLIGOS
                    start  len      tm     gc%  any_th  3'_th hairpin seq

 1 LEFT PRIMER         10   20   59.70   50.00    0.00   0.00    0.00 TGACTTCACTTTCCGACCCT
   RIGHT PRIMER       113   20   59.96   50.00    9.59   0.00    0.00 TGACGGTCATCATGGTGAGT
   INTERNAL OLIGO      44   20   60.71   60.00    0.00   0.00   40.53 CACCCACGAGTGAAGACTCC
   PRODUCT SIZE: 104, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 2 LEFT PRIMER         11   20   59.70   55.00    0.00   0.00    0.00 GACTTCACTTTCCGACCCTG
   RIGHT PRIMER       114   20   60.11   55.00    9.19   0.00    0.00 CTGACGGTCATCATGGTGAG
   INTERNAL OLIGO      44   20   60.71   60.00    0.00   0.00   40.53 CACCCACGAGTGAAGACTCC
   PRODUCT SIZE: 104, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 3 LEFT PRIMER         10   20   59.70   50.00    0.00   0.00    0.00 TGACTTCACTTTCCGACCCT
   RIGHT PRIMER       114   20   60.11   55.00    9.19   0.00    0.00 CTGACGGTCATCATGGTGAG
   INTERNAL OLIGO      44   20   60.71   60.00    0.00   0.00   40.53 CACCCACGAGTGAAGACTCC
   PRODUCT SIZE: 105, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 4 LEFT PRIMER          2   20   59.29   50.00    0.00   0.00    0.00 GCCGAGGATGACTTCACTTT
   RIGHT PRIMER       113   20   59.96   50.00    9.59   0.00    0.00 TGACGGTCATCATGGTGAGT
   INTERNAL OLIGO      44   20   60.71   60.00    0.00   0.00   40.53 CACCCACGAGTGAAGACTCC
   PRODUCT SIZE: 112, PAIR ANY_TH COMPL: 8.76, PAIR 3'_TH COMPL: 2.04

Statistics
         con   too    in    in   not          no    tm    tm   high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too any_th 3'_th hair-  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high  compl compl   pin     X  stab    ok
Left    1290     0     0     0     0    10     0    60  1039      0     0     0     0     0   181
Right   1290     0     0     0     0    32     0    38  1096      0     0     0     0     0   124
Intl    1565     0     0     0     0    32     0    60  1290      0     0     0     0     0   183
Pair Stats:
considered 25, unacceptable product size 20, ok 5
libprimer3 release 2.3.5


PRIMER PICKING RESULTS FOR any_1

No mispriming library specified
No internal oligo mishyb library specified
Using 0-based sequence positions
OLIGO            start  len      tm     gc%  any_th  3'_th hairpin seq
LEFT PRIMER         17   20   60.01   50.00    2.42   0.00   42.83 CAAGCAATACGACCTGCTGA
RIGHT PRIMER       168   20   59.98   50.00    0.00   0.00   39.48 ATCGCAAGTGAGGATGAGCT
INTERNAL OLIGO     116   21   60.58   38.10   12.25  10.49   37.82 CGTGTGCAAAACATTTTCACA
SEQUENCE SIZE: 404
INCLUDED REGION SIZE: 404

PRODUCT SIZE: 152, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

    0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC
                       >>>>>>>>>>>>>>>>>>>>                       

   60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG
                                                              ^^^^

  120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC
      ^^^^^^^^^^^^^^^^^            <<<<<<<<<<<<<<<<<<<<           

  180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT
                                                                  

  240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC
                                                                  

  300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG
                                                                  

  360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
                                                  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo

ADDITIONAL OLIGOS
                    start  len      tm     gc%  any_th  3'_th hairpin seq

 1 LEFT PRIMER         17   20   60.01   50.00    2.42   0.00   42.83 CAAGCAATACGACCTGCTGA
   RIGHT PRIMER       191   20   59.94   40.00    5.65   0.00   39.80 CAAAAGATTTTGGCGGGTAA
   INTERNAL OLIGO     149   20   59.98   50.00    0.00   0.00    0.00 AGCTCATCCTCACTTGCGAT
   PRODUCT SIZE: 175, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 2 LEFT PRIMER         17   20   60.01   50.00    2.42   0.00   42.83 CAAGCAATACGACCTGCTGA
   RIGHT PRIMER       368   20   59.75   60.00    8.58   0.00    0.00 CCGTACGCACTATAGGAGCC
   INTERNAL OLIGO     149   20   59.98   50.00    0.00   0.00    0.00 AGCTCATCCTCACTTGCGAT
   PRODUCT SIZE: 352, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 3 LEFT PRIMER        149   20   59.98   50.00    0.00   0.00    0.00 AGCTCATCCTCACTTGCGAT
   RIGHT PRIMER       368   20   59.75   60.00    8.58   0.00    0.00 CCGTACGCACTATAGGAGCC
   INTERNAL OLIGO     172   20   59.94   40.00    4.21   4.21   43.62 TTACCCGCCAAAATCTTTTG
   PRODUCT SIZE: 220, PAIR ANY_TH COMPL: 7.44, PAIR 3'_TH COMPL: 0.00

 4 LEFT PRIMER         17   20   60.01   50.00    2.42   0.00   42.83 CAAGCAATACGACCTGCTGA
   RIGHT PRIMER       232   20   59.70   50.00    0.00   0.00    0.00 CACTAAAGCGGGATTTGAGG
   INTERNAL OLIGO     149   20   59.98   50.00    0.00   0.00    0.00 AGCTCATCCTCACTTGCGAT
   PRODUCT SIZE: 216, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

Statistics
         con   too    in    in   not          no    tm    tm   high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too any_th 3'_th hair-  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high  compl compl   pin     X  stab    ok
Left    3397     0     0     0     0     0     0   897  1543      0     0     0    22     0   935
Right   3397     0     0     0     0     0     0   839  1673      0     0     1    22     0   862
Intl    3672     0     0     0     0     0     0   953  1734      0     0    14    22     0   949
Pair Stats:
considered 58, unacceptable product size 51, ok 7
libprimer3 release 2.3.5


PRIMER PICKING RESULTS FOR any_salt

No mispriming library specified
No internal oligo mishyb library specified
Using 0-based sequence positions
WARNING: PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC

OLIGO            start  len      tm     gc%  any_th  3'_th hairpin seq
LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
SEQUENCE SIZE: 404
INCLUDED REGION SIZE: 404

PRODUCT SIZE: 157, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

    0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC
                                                                  

   60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG
                                            >>>>>>>>>>>>>>>>>>>>  

  120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC
                               ^^^^^^^^^^^^^^^^^^^^               

  180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT
                                                             <<<<<

  240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC
      <<<<<<<<<<<<<<<                                             

  300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG
                                                                  

  360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
                                                  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo

ADDITIONAL OLIGOS
                    start  len      tm     gc%  any_th  3'_th hairpin seq

 1 LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 146, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 2 LEFT PRIMER        224   20   60.39   45.00    0.00   0.00    0.00 GCTTTAGTGTAGTAGTGAGC
   RIGHT PRIMER       401   20   61.03   40.00    0.00   0.00    0.00 ATACTGATGTCTATAGTGCG
   INTERNAL OLIGO     340   20   59.83   45.00    0.00   0.00    0.00 GCAGAAAAAGGCTCCTATAG
   PRODUCT SIZE: 178, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 3 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 156, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 4 LEFT PRIMER        103   19   59.90   42.11    2.59   0.51    0.00 TCTATCTGTACAACGTGTG
   RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 152, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

Statistics
         con   too    in    in   not          no    tm    tm   high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too any_th 3'_th hair-  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high  compl compl   pin     X  stab    ok
Left    3397     0     0     0     0     0     0    52  3139      0     0     1    22     0   183
Right   3397     0     0     0     0     0     0    59  3137      0     0     0    22     0   179
Intl    3672     0     0     0     0     0     0   432  2545      0     0    10    22     0   663
Pair Stats:
considered 15, unacceptable product size 10, ok 5
libprimer3 release 2.3.5


PRIMER PICKING RESULTS FOR any_weight

No mispriming library specified
No internal oligo mishyb library specified
Using 0-based sequence positions
WARNING: PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC

OLIGO            start  len      tm     gc%  any_th  3'_th hairpin seq
LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
SEQUENCE SIZE: 404
INCLUDED REGION SIZE: 404

PRODUCT SIZE: 157, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

    0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC
                                                                  

   60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG
                                            >>>>>>>>>>>>>>>>>>>>  

  120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC
                               ^^^^^^^^^^^^^^^^^^^^               

  180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT
                                                             <<<<<

  240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC
      <<<<<<<<<<<<<<<                                             

  300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG
                                                                  

  360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
                                                  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo

ADDITIONAL OLIGOS
                    start  len      tm     gc%  any_th  3'_th hairpin seq

 1 LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 146, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 2 LEFT PRIMER        224   20   60.39   45.00    0.00   0.00    0.00 GCTTTAGTGTAGTAGTGAGC
   RIGHT PRIMER       401   20   61.03   40.00    0.00   0.00    0.00 ATACTGATGTCTATAGTGCG
   INTERNAL OLIGO     340   20   59.83   45.00    0.00   0.00    0.00 GCAGAAAAAGGCTCCTATAG
   PRODUCT SIZE: 178, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 3 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 156, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 4 LEFT PRIMER        103   19   59.90   42.11    2.59   0.51    0.00 TCTATCTGTACAACGTGTG
   RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 152, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

Statistics
         con   too    in    in   not          no    tm    tm   high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too any_th 3'_th hair-  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high  compl compl   pin     X  stab    ok
Left    3397     0     0     0     0     0     0    52  3139      0     0     1    22     0   183
Right   3397     0     0     0     0     0     0    59  3137      0     0     0    22     0   179
Intl    3672     0     0     0     0     0     0   432  2545      0     0    10    22     0   663
Pair Stats:
considered 15, unacceptable product size 10, ok 5
libprimer3 release 2.3.5


PRIMER PICKING RESULTS FOR any_2

No mispriming library specified
No internal oligo mishyb library specified
Using 0-based sequence positions
WARNING: PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC

OLIGO            start  len      tm     gc%  any_th  3'_th hairpin seq
LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
SEQUENCE SIZE: 404
INCLUDED REGION SIZE: 404

PRODUCT SIZE: 157, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

    0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC
                                                                  

   60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG
                                            >>>>>>>>>>>>>>>>>>>>  

  120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC
                               ^^^^^^^^^^^^^^^^^^^^               

  180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT
                                                             <<<<<

  240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC
      <<<<<<<<<<<<<<<                                             

  300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG
                                                                  

  360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
                                                  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo

ADDITIONAL OLIGOS
                    start  len      tm     gc%  any_th  3'_th hairpin seq

 1 LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 146, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 2 LEFT PRIMER        224   20   60.39   45.00    0.00   0.00    0.00 GCTTTAGTGTAGTAGTGAGC
   RIGHT PRIMER       401   20   61.03   40.00    0.00   0.00    0.00 ATACTGATGTCTATAGTGCG
   INTERNAL OLIGO     340   20   59.83   45.00    0.00   0.00    0.00 GCAGAAAAAGGCTCCTATAG
   PRODUCT SIZE: 178, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 3 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 156, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 4 LEFT PRIMER        103   19   59.90   42.11    2.59   0.51    0.00 TCTATCTGTACAACGTGTG
   RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 152, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

Statistics
         con   too    in    in   not          no    tm    tm   high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too any_th 3'_th hair-  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high  compl compl   pin     X  stab    ok
Left    3155     0     0     0     0     0     0    52  2924     32     0     1    22     0   124
Right   3213     0     0     0     0     0     0    59  2968     23     0     0    19     0   144
Intl    3185     0     0     0     0     0     0   432  2188     82     0    10    22     0   451
Pair Stats:
considered 15, unacceptable product size 10, ok 5
libprimer3 release 2.3.5


PRIMER PICKING RESULTS FOR any_no_effect

No mispriming library specified
No internal oligo mishyb library specified
Using 0-based sequence positions
WARNING: PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC

OLIGO            start  len      tm     gc%  any_th  3'_th hairpin seq
LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
SEQUENCE SIZE: 404
INCLUDED REGION SIZE: 404

PRODUCT SIZE: 157, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

    0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC
                                                                  

   60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG
                                            >>>>>>>>>>>>>>>>>>>>  

  120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC
                               ^^^^^^^^^^^^^^^^^^^^               

  180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT
                                                             <<<<<

  240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC
      <<<<<<<<<<<<<<<                                             

  300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG
                                                                  

  360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
                                                  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo

ADDITIONAL OLIGOS
                    start  len      tm     gc%  any_th  3'_th hairpin seq

 1 LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 146, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 2 LEFT PRIMER        224   20   60.39   45.00    0.00   0.00    0.00 GCTTTAGTGTAGTAGTGAGC
   RIGHT PRIMER       401   20   61.03   40.00    0.00   0.00    0.00 ATACTGATGTCTATAGTGCG
   INTERNAL OLIGO     340   20   59.83   45.00    0.00   0.00    0.00 GCAGAAAAAGGCTCCTATAG
   PRODUCT SIZE: 178, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 3 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 156, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 4 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 145, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

Statistics
         con   too    in    in   not          no    tm    tm   high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too any_th 3'_th hair-  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high  compl compl   pin     X  stab    ok
Left    3397     0     0     0     0     0     0    52  3139      1     0     1    22     0   182
Right   3397     0     0     0     0     0     0    59  3137      0     0     0    22     0   179
Intl    3672     0     0     0     0     0     0   432  2545     11     0     4    22     0   658
Pair Stats:
considered 17, unacceptable product size 11, ok 6
libprimer3 release 2.3.5


PRIMER PICKING RESULTS FOR end_th1

No mispriming library specified
No internal oligo mishyb library specified
Using 0-based sequence positions
WARNING: PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC

OLIGO            start  len      tm     gc%  any_th  3'_th hairpin seq
LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
SEQUENCE SIZE: 404
INCLUDED REGION SIZE: 404

PRODUCT SIZE: 157, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

    0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC
                                                                  

   60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG
                                            >>>>>>>>>>>>>>>>>>>>  

  120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC
                               ^^^^^^^^^^^^^^^^^^^^               

  180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT
                                                             <<<<<

  240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC
      <<<<<<<<<<<<<<<                                             

  300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG
                                                                  

  360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
                                                  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo

ADDITIONAL OLIGOS
                    start  len      tm     gc%  any_th  3'_th hairpin seq

 1 LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 146, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 2 LEFT PRIMER        224   20   60.39   45.00    0.00   0.00    0.00 GCTTTAGTGTAGTAGTGAGC
   RIGHT PRIMER       401   20   61.03   40.00    0.00   0.00    0.00 ATACTGATGTCTATAGTGCG
   INTERNAL OLIGO     340   20   59.83   45.00    0.00   0.00    0.00 GCAGAAAAAGGCTCCTATAG
   PRODUCT SIZE: 178, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 3 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 156, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 4 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 145, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

Statistics
         con   too    in    in   not          no    tm    tm   high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too any_th 3'_th hair-  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high  compl compl   pin     X  stab    ok
Left    3397     0     0     0     0     0     0    52  3139      1     0     1    22     0   182
Right   3397     0     0     0     0     0     0    59  3137      0     0     0    22     0   179
Intl    3672     0     0     0     0     0     0   432  2545     11     0     4    22     0   658
Pair Stats:
considered 17, unacceptable product size 11, ok 6
libprimer3 release 2.3.5


PRIMER PICKING RESULTS FOR end_th2

No mispriming library specified
No internal oligo mishyb library specified
Using 0-based sequence positions
WARNING: PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC

OLIGO            start  len      tm     gc%  any_th  3'_th hairpin seq
LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
SEQUENCE SIZE: 404
INCLUDED REGION SIZE: 404

PRODUCT SIZE: 157, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

    0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC
                                                                  

   60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG
                                            >>>>>>>>>>>>>>>>>>>>  

  120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC
                               ^^^^^^^^^^^^^^^^^^^^               

  180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT
                                                             <<<<<

  240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC
      <<<<<<<<<<<<<<<                                             

  300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG
                                                                  

  360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
                                                  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo

ADDITIONAL OLIGOS
                    start  len      tm     gc%  any_th  3'_th hairpin seq

 1 LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 146, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 2 LEFT PRIMER        224   20   60.39   45.00    0.00   0.00    0.00 GCTTTAGTGTAGTAGTGAGC
   RIGHT PRIMER       401   20   61.03   40.00    0.00   0.00    0.00 ATACTGATGTCTATAGTGCG
   INTERNAL OLIGO     340   20   59.83   45.00    0.00   0.00    0.00 GCAGAAAAAGGCTCCTATAG
   PRODUCT SIZE: 178, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 3 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 156, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 4 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 145, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

Statistics
         con   too    in    in   not          no    tm    tm   high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too any_th 3'_th hair-  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high  compl compl   pin     X  stab    ok
Left    3075     0     0     0     0     0     0    52  2860     42     0     0    22     0    99
Right   3118     0     0     0     0     0     0    59  2890     38     0     0    17     0   114
Intl    2916     0     0     0     0     0     0   432  1999    116     0     4    22     0   343
Pair Stats:
considered 14, unacceptable product size 5, ok 9
libprimer3 release 2.3.5


PRIMER PICKING RESULTS FOR hairpin1

No mispriming library specified
No internal oligo mishyb library specified
Using 0-based sequence positions
WARNING: PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC

OLIGO            start  len      tm     gc%  any_th  3'_th hairpin seq
LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
SEQUENCE SIZE: 404
INCLUDED REGION SIZE: 404

PRODUCT SIZE: 157, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

    0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC
                                                                  

   60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG
                                            >>>>>>>>>>>>>>>>>>>>  

  120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC
                               ^^^^^^^^^^^^^^^^^^^^               

  180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT
                                                             <<<<<

  240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC
      <<<<<<<<<<<<<<<                                             

  300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG
                                                                  

  360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
                                                  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo

ADDITIONAL OLIGOS
                    start  len      tm     gc%  any_th  3'_th hairpin seq

 1 LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 146, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 2 LEFT PRIMER        224   20   60.39   45.00    0.00   0.00    0.00 GCTTTAGTGTAGTAGTGAGC
   RIGHT PRIMER       401   20   61.03   40.00    0.00   0.00    0.00 ATACTGATGTCTATAGTGCG
   INTERNAL OLIGO     340   20   59.83   45.00    0.00   0.00    0.00 GCAGAAAAAGGCTCCTATAG
   PRODUCT SIZE: 178, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 3 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 156, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 4 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 145, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

Statistics
         con   too    in    in   not          no    tm    tm   high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too any_th 3'_th hair-  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high  compl compl   pin     X  stab    ok
Left    3075     0     0     0     0     0     0    52  2860     42     0     0    22     0    99
Right   3118     0     0     0     0     0     0    59  2890     38     0     0    17     0   114
Intl    2916     0     0     0     0     0     0   432  1999    116     0     4    22     0   343
Pair Stats:
considered 14, unacceptable product size 5, ok 9
libprimer3 release 2.3.5


PRIMER PICKING RESULTS FOR hairpin2

No mispriming library specified
No internal oligo mishyb library specified
Using 0-based sequence positions
WARNING: PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC

OLIGO            start  len      tm     gc%  any_th  3'_th hairpin seq
LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
SEQUENCE SIZE: 404
INCLUDED REGION SIZE: 404

PRODUCT SIZE: 157, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

    0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC
                                                                  

   60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG
                                            >>>>>>>>>>>>>>>>>>>>  

  120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC
                               ^^^^^^^^^^^^^^^^^^^^               

  180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT
                                                             <<<<<

  240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC
      <<<<<<<<<<<<<<<                                             

  300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG
                                                                  

  360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
                                                  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo

ADDITIONAL OLIGOS
                    start  len      tm     gc%  any_th  3'_th hairpin seq

 1 LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 146, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 2 LEFT PRIMER        224   20   60.39   45.00    0.00   0.00    0.00 GCTTTAGTGTAGTAGTGAGC
   RIGHT PRIMER       401   20   61.03   40.00    0.00   0.00    0.00 ATACTGATGTCTATAGTGCG
   INTERNAL OLIGO     340   20   59.83   45.00    0.00   0.00    0.00 GCAGAAAAAGGCTCCTATAG
   PRODUCT SIZE: 178, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 3 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 156, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 4 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 145, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

Statistics
         con   too    in    in   not          no    tm    tm   high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too any_th 3'_th hair-  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high  compl compl   pin     X  stab    ok
Left    3075     0     0     0     0     0     0    52  2860     42     0     0    22     0    99
Right   3118     0     0     0     0     0     0    59  2890     38     0     0    17     0   114
Intl    2916     0     0     0     0     0     0   432  1999    116     0     4    22     0   343
Pair Stats:
considered 14, unacceptable product size 5, ok 9
libprimer3 release 2.3.5


PRIMER PICKING RESULTS FOR hairpin3

No mispriming library specified
No internal oligo mishyb library specified
Using 0-based sequence positions
WARNING: PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC

OLIGO            start  len      tm     gc%  any_th  3'_th hairpin seq
LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
SEQUENCE SIZE: 404
INCLUDED REGION SIZE: 404

PRODUCT SIZE: 157, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

    0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC
                                                                  

   60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG
                                            >>>>>>>>>>>>>>>>>>>>  

  120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC
                               ^^^^^^^^^^^^^^^^^^^^               

  180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT
                                                             <<<<<

  240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC
      <<<<<<<<<<<<<<<                                             

  300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG
                                                                  

  360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
                                                  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo

ADDITIONAL OLIGOS
                    start  len      tm     gc%  any_th  3'_th hairpin seq

 1 LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 146, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 2 LEFT PRIMER        224   20   60.39   45.00    0.00   0.00    0.00 GCTTTAGTGTAGTAGTGAGC
   RIGHT PRIMER       401   20   61.03   40.00    0.00   0.00    0.00 ATACTGATGTCTATAGTGCG
   INTERNAL OLIGO     340   20   59.83   45.00    0.00   0.00    0.00 GCAGAAAAAGGCTCCTATAG
   PRODUCT SIZE: 178, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 3 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 156, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 4 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 145, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

Statistics
         con   too    in    in   not          no    tm    tm   high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too any_th 3'_th hair-  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high  compl compl   pin     X  stab    ok
Left    3075     0     0     0     0     0     0    52  2860     42     0     0    22     0    99
Right   3118     0     0     0     0     0     0    59  2890     38     0     0    17     0   114
Intl    2916     0     0     0     0     0     0   432  1999    116     0    22    22     0   325
Pair Stats:
considered 14, unacceptable product size 5, ok 9
libprimer3 release 2.3.5


PRIMER PICKING RESULTS FOR template1

No mispriming library specified
No internal oligo mishyb library specified
Using 0-based sequence positions
WARNING: PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC

OLIGO            start  len      tm     gc%  any_th  3'_th hairpin seq
LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
SEQUENCE SIZE: 404
INCLUDED REGION SIZE: 404

PRODUCT SIZE: 157, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

    0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC
                                                                  

   60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG
                                            >>>>>>>>>>>>>>>>>>>>  

  120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC
                               ^^^^^^^^^^^^^^^^^^^^               

  180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT
                                                             <<<<<

  240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC
      <<<<<<<<<<<<<<<                                             

  300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG
                                                                  

  360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
                                                  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo

ADDITIONAL OLIGOS
                    start  len      tm     gc%  any_th  3'_th hairpin seq

 1 LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 146, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 2 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 156, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 3 LEFT PRIMER        224   20   60.39   45.00    0.00   0.00    0.00 GCTTTAGTGTAGTAGTGAGC
   RIGHT PRIMER       401   20   61.03   40.00    0.00   0.00    0.00 ATACTGATGTCTATAGTGCG
   INTERNAL OLIGO     340   20   59.83   45.00    0.00   0.00    0.00 GCAGAAAAAGGCTCCTATAG
   PRODUCT SIZE: 178, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 4 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 145, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

Statistics
         con   too    in    in   not          no    tm    tm   high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too any_th 3'_th hair-  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high  compl compl   pin     X  stab    ok
Left    3075     0     0     0     0     0     0    52  2860     42     0     0    22     0    99
Right   3118     0     0     0     0     0     0    59  2890     38     0     0    17     0   114
Intl    2916     0     0     0     0     0     0   432  1999    116     0    22    22     0   325
Pair Stats:
considered 14, unacceptable product size 5, ok 9
libprimer3 release 2.3.5


PRIMER PICKING RESULTS FOR template2

No mispriming library specified
No internal oligo mishyb library specified
Using 0-based sequence positions
WARNING: PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC

OLIGO            start  len      tm     gc%  any_th  3'_th hairpin seq
LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
SEQUENCE SIZE: 404
INCLUDED REGION SIZE: 404

PRODUCT SIZE: 157, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

    0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC
                                                                  

   60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG
                                            >>>>>>>>>>>>>>>>>>>>  

  120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC
                               ^^^^^^^^^^^^^^^^^^^^               

  180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT
                                                             <<<<<

  240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC
      <<<<<<<<<<<<<<<                                             

  300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG
                                                                  

  360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
                                                  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo

ADDITIONAL OLIGOS
                    start  len      tm     gc%  any_th  3'_th hairpin seq

 1 LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 ATTTTTCTATCTGTACAACG
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 146, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 2 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 GACCTGTAATAGCTCACTAC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 156, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 3 LEFT PRIMER        224   20   60.39   45.00    0.00   0.00    0.00 GCTTTAGTGTAGTAGTGAGC
   RIGHT PRIMER       401   20   61.03   40.00    0.00   0.00    0.00 ATACTGATGTCTATAGTGCG
   INTERNAL OLIGO     340   20   59.83   45.00    0.00   0.00    0.00 GCAGAAAAAGGCTCCTATAG
   PRODUCT SIZE: 178, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 4 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00 AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 145, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

Statistics
         con   too    in    in   not          no    tm    tm   high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too any_th 3'_th hair-  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high  compl compl   pin     X  stab    ok
Left    3075     0     0     0     0     0     0    52  2860     42     0     0    22     0    99
Right   3118     0     0     0     0     0     0    59  2890     38     0     0    17     0   114
Intl    2916     0     0     0     0     0     0   432  1999    116     0    22    22     0   325
Pair Stats:
considered 14, unacceptable product size 5, ok 9
libprimer3 release 2.3.5


PRIMER PICKING RESULTS FOR template2_and_repeat_library

Using mispriming library ../src/humrep_and_simple.txt
No internal oligo mishyb library specified
Using 0-based sequence positions
WARNING: PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC

OLIGO            start  len      tm     gc%  any_th  3'_th hairpin   rep seq
LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 12.00 ATTTTTCTATCTGTACAACG
RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 11.00 GACCTGTAATAGCTCACTAC
INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00       AAACAGCTCATCCTCACTTG
SEQUENCE SIZE: 404
INCLUDED REGION SIZE: 404

PRODUCT SIZE: 157, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

    0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC
                                                                  

   60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG
                                            >>>>>>>>>>>>>>>>>>>>  

  120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC
                               ^^^^^^^^^^^^^^^^^^^^               

  180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT
                                                             <<<<<

  240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC
      <<<<<<<<<<<<<<<                                             

  300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG
                                                                  

  360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
                                                  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo

ADDITIONAL OLIGOS
                    start  len      tm     gc%  any_th  3'_th hairpin   rep seq

 1 LEFT PRIMER         98   20   60.84   30.00    0.00   0.00    0.00 12.00 ATTTTTCTATCTGTACAACG
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 12.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00       AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 146, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 2 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 11.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       254   20   59.66   45.00    0.00   0.00    0.00 11.00 GACCTGTAATAGCTCACTAC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00       AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 156, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 3 LEFT PRIMER        224   20   60.39   45.00    0.00   0.00    0.00 11.00 GCTTTAGTGTAGTAGTGAGC
   RIGHT PRIMER       401   20   61.03   40.00    0.00   0.00    0.00 12.00 ATACTGATGTCTATAGTGCG
   INTERNAL OLIGO     340   20   59.83   45.00    0.00   0.00    0.00       GCAGAAAAAGGCTCCTATAG
   PRODUCT SIZE: 178, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 4 LEFT PRIMER         99   20   61.09   30.00    0.00   0.00    0.00 11.00 TTTTTCTATCTGTACAACGT
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 12.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO     145   20   59.87   45.00    0.00   0.00    0.00       AAACAGCTCATCCTCACTTG
   PRODUCT SIZE: 145, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

Statistics
         con   too    in    in   not          no    tm    tm   high  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too any_th 3'_th hair-   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high  compl compl   pin   sim     X  stab    ok
Left    3075     0     0     0     0     0     0    52  2860     42     0     0     0    22     0    99
Right   3118     0     0     0     0     0     0    59  2890     38     0     0     0    17     0   114
Intl    2916     0     0     0     0     0     0   432  1999    116     0    22     0    22     0   325
Pair Stats:
considered 14, unacceptable product size 5, ok 9
libprimer3 release 2.3.5


PRIMER PICKING RESULTS FOR template3

Using mispriming library ../src/humrep_and_simple.txt
No internal oligo mishyb library specified
Using 0-based sequence positions
WARNING: PRIMER_SALT_DIVALENT > 0.0 but PRIMER_DNTP_CONC <= 0.0; use reasonable value for PRIMER_DNTP_CONC

OLIGO            start  len      tm     gc%  any_th  3'_th hairpin   rep seq
LEFT PRIMER          8   19   59.84   31.58    0.00   0.00    0.00 12.00 CATTCAATACAAGCAATAC
RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 12.00 GCTCACTACTACACTAAAGC
INTERNAL OLIGO      72   20   59.96   45.00    0.00   0.00    0.00       GCTCATTAAGACCGTACGAT
SEQUENCE SIZE: 404
INCLUDED REGION SIZE: 404

PRODUCT SIZE: 236, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

    0 AGTGAGCTCATTCAATACAAGCAATACGACCTGCTGATTATCGACGATATTCAGTTCATC
              >>>>>>>>>>>>>>>>>>>                                 

   60 AAAGGAAAGTGAGCTCATTAAGACCGTACGATGGAAGAATTTTTCTATCTGTACAACGTG
                  ^^^^^^^^^^^^^^^^^^^^                            

  120 TGCAAAACATTTTCACAATGAGAAAAAACAGCTCATCCTCACTTGCGATGTTTTACCCGC
                                                                  

  180 CAAAATCTTTTGCACCGAAGGTATGGACGACCGCCTCAAATCCCGCTTTAGTGTAGTAGT
                                                  <<<<<<<<<<<<<<<<

  240 GAGCTATTACAGGTCGTGGGGGCTGACCGCAGTAAGCTGCGGCTTTGGAACTCGAGCCGC
      <<<<                                                        

  300 CCGAATTGGAAATGCGCAAAACCGTGTCTATCGCCATTTTGCAGAAAAAGGCTCCTATAG
                                                                  

  360 TGCGTACGGAAGCGGCGGGGTACGCACTATAGACATCAGTATCG
                                                  

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo

ADDITIONAL OLIGOS
                    start  len      tm     gc%  any_th  3'_th hairpin   rep seq

 1 LEFT PRIMER         46   19   60.82   31.58    0.00   0.00    0.00 10.00 ATATTCAGTTCATCAAAGG
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 12.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO      72   20   59.96   45.00    0.00   0.00    0.00       GCTCATTAAGACCGTACGAT
   PRODUCT SIZE: 198, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 2 LEFT PRIMER          8   19   59.84   31.58    0.00   0.00    0.00 12.00 CATTCAATACAAGCAATAC
   RIGHT PRIMER       241   19   59.75   42.11    0.00   0.00    0.00 11.00 TCACTACTACACTAAAGCG
   INTERNAL OLIGO      72   20   59.96   45.00    0.00   0.00    0.00       GCTCATTAAGACCGTACGAT
   PRODUCT SIZE: 234, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 3 LEFT PRIMER          7   20   62.06   30.00    0.00   0.00    0.00 12.00 TCATTCAATACAAGCAATAC
   RIGHT PRIMER       243   20   60.39   45.00    0.00   0.00    0.00 12.00 GCTCACTACTACACTAAAGC
   INTERNAL OLIGO      72   20   59.96   45.00    0.00   0.00    0.00       GCTCATTAAGACCGTACGAT
   PRODUCT SIZE: 237, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

 4 LEFT PRIMER          8   19   59.84   31.58    0.00   0.00    0.00 12.00 CATTCAATACAAGCAATAC
   RIGHT PRIMER       242   20   61.41   45.00    0.00   0.00    0.00 12.00 CTCACTACTACACTAAAGCG
   INTERNAL OLIGO      72   20   59.96   45.00    0.00   0.00    0.00       GCTCATTAAGACCGTACGAT
   PRODUCT SIZE: 235, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

Statistics
         con   too    in    in   not          no    tm    tm   high  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too any_th 3'_th hair-   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high  compl compl   pin   sim     X  stab    ok
Left    3075     0     0     0     0     0     0    52  2860     42     0    18     3    22     0    78
Right   3118     0     0     0     0     0     0    59  2890     38     0    25     0    17     0    89
Intl    2916     0     0     0     0     0     0   432  1999    116     0    22     0    22     0   325
Pair Stats:
considered 7017, unacceptable product size 2861, high any compl 150, no internal oligo 210, high template mispriming score 97, ok 3699
libprimer3 release 2.3.5


