SEQUENCE_ID=test of PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=-1 - 11 possible pairs
PRIMER_PRODUCT_SIZE_RANGE=100-500
PRIMER_MIN_TM=0
PRIMER_MAX_TM=100
PRIMER_NUM_RETURN=40
PRIMER_MAX_SELF_ANY=100
PRIMER_MAX_SELF_END=100
PRIMER_PAIR_MAX_COMPL_ANY=100
PRIMER_PAIR_MAX_COMPL_END=100
PRIMER_MIN_SIZE=20
PRIMER_MAX_SIZE=20
SEQUENCE_PRIMER=ATGAATTAGCAAGTCATTAT
SEQUENCE_EXCLUDED_REGION=21,89
PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=-1
SEQUENCE_TEMPLATE=ATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCC
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SEQUENCE_ID=test of PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=0 - same results as for -1
SEQUENCE_PRIMER=ATGAATTAGCAAGTCATTAT
SEQUENCE_EXCLUDED_REGION=21,89
PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=0
SEQUENCE_TEMPLATE=ATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCC
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SEQUENCE_ID=test of PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=1 - same results as for 0 and -1
SEQUENCE_PRIMER=ATGAATTAGCAAGTCATTAT
SEQUENCE_EXCLUDED_REGION=21,89
PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=1
SEQUENCE_TEMPLATE=ATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCC
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SEQUENCE_ID=test of PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=2 - 5 pairs
SEQUENCE_PRIMER=ATGAATTAGCAAGTCATTAT
SEQUENCE_EXCLUDED_REGION=21,89
PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=2
SEQUENCE_TEMPLATE=ATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCC
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SEQUENCE_ID=test of PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=3 - 4 pairs
SEQUENCE_PRIMER=ATGAATTAGCAAGTCATTAT
SEQUENCE_EXCLUDED_REGION=21,89
PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=3
SEQUENCE_TEMPLATE=ATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTCTGAGCAAAGTCTCTTGCAAATCCCC
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SEQUENCE_ID=test of PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=-1 - 11 possible pairs
SEQUENCE_PRIMER_REVCOMP=TTTCACTTTCTAATGAAGGC
SEQUENCE_EXCLUDED_REGION=31,89
PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=-1
SEQUENCE_TEMPLATE=TTTAGATATGATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCCTTCATTAGAAAGTGAAA
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SEQUENCE_ID=test of PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=0 - same results as for -1
SEQUENCE_PRIMER_REVCOMP=TTTCACTTTCTAATGAAGGC
SEQUENCE_EXCLUDED_REGION=31,89
PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=0
SEQUENCE_TEMPLATE=TTTAGATATGATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCCTTCATTAGAAAGTGAAA
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SEQUENCE_ID=test of PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=1 - same results as for 0 and -1
SEQUENCE_PRIMER_REVCOMP=TTTCACTTTCTAATGAAGGC
SEQUENCE_EXCLUDED_REGION=31,89
PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=1
SEQUENCE_TEMPLATE=TTTAGATATGATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCCTTCATTAGAAAGTGAAA
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SEQUENCE_ID=test of PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=2 - 5 pairs
SEQUENCE_PRIMER_REVCOMP=TTTCACTTTCTAATGAAGGC
SEQUENCE_EXCLUDED_REGION=31,89
PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=2
SEQUENCE_TEMPLATE=TTTAGATATGATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCCTTCATTAGAAAGTGAAA
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SEQUENCE_ID=test of PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=3 - 3 pairs
SEQUENCE_PRIMER_REVCOMP=TTTCACTTTCTAATGAAGGC
SEQUENCE_EXCLUDED_REGION=31,89
PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=3
SEQUENCE_TEMPLATE=TTTAGATATGATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCCTTCATTAGAAAGTGAAA
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